AnnexinV (endonexin II, lipocortin V, 35K calelectrin, 35-g-calcimedin, PP4, IBC, PAP-I, VAC-a, calphobindin I, anchorin CII, thromboblastin inhibitor)

Flow cytometry

Flow cytometry is a technique for counting, examining, and sorting microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus.

The annexin V assay has been widely accepted as a marker of apoptosis
more info would be given in near future.

Platelets

Platelets, or thrombocytes, are the cells circulating in the blood that are involved in the cellular mechanisms of primary hemostasis leading to the formation of blood clots. In strict sense, the term platelet should be reserved for anucleated thrombocytes of mammals. Nucleated thrombocytes of nonmammalian vertebrates differ from the mammalian plates not only in having a nucleus and thus resembling B lymphocytes, but also these nucleated thrombocytes do not aggregate in response to ADP, serotonin and adrenaline (they do aggregate with thrombin, of course.) [1]. Dysfunction or low levels of platelets predisposes to bleeding, while high levels, although usually asymptomatic, may increase the risk of thrombosis. An abnormality or disease of the platelets is called a thrombocytopathy[2] which could be either a decrease in number ie thrombocytopenia, decrease in function ie thrombasthenia or an increase in number ie thrombocytosis

Annexin 5

To day we had a meeting with Dr Vickers , supervisior of project . We have been discussing about the project which is supposed to be done in this year.
At the end , while I did not want but I asked him, could we publish it? he said I have not already published the projects of students but it is good , perhaps!
now pray for me! if I can publish it it would be a real vistory!!!!!!!

Definition of Annexin V
Annexin V: Annexin V is the cause of a syndrome called the antiphospholipid antibody syndrome with abnormal blood clotting.
The annexins are a family of proteins first described in 1990. All of the annexin proteins share the property of binding calcium and phospholipids.
Annexin V normally forms a shield around certain phospholipid molecules that blocks their entry into coagulation (clotting) reactions. In the antiphospholipid antibody syndrome, the formation of this shield is disrupted by the abnormal antibodies. Without the shield, there is an increased quantity of phospholipid molecules on cell membranes, speeding up coagulation reactions and causing the abnormal blood clotting characteristic of the antiphospholipid antibody syndrome

April 2008

Blood Transfusion Journal -April 2008
please click here .